Generator

Part:BBa_K1875011:Design

Designed by: Jeffrey Marano   Group: iGEM16_BostonU   (2016-10-11)


This part produces a guide RNA that pairs with an operator.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part produces the guide in BBa_K1875004 and the guide that matches the target sequence on BBa_K1875014

Source

This plasmid was based off of the work by Feng Zhang at MIT and the x330 plasmid.

References